How can we Improve Pipetting Accuracy?
When you dispense liquid from your pipette, a coating of liquid is left on the tip, which makes the dispensed volume slightly less than that displayed on the pipette. Pre-wetting the tip before pipetting will help increase accuracy in your results. To pre-wet your tip, draw up the liquid into your pipette and then dispense it back into the original vessel. Here are the tips to pipette accurately, especially small volumes:
https://www.microlit.com/pipetting-small-volumes-8-tips-to-improve-your-technique/